DYAH PERWITASARI-FARAJALLAH1,2,♥, RANDALL C. KYES1,3, ENTANG ISKANDAR1

1Primate Research Center, Bogor Agricultural University (IPB), Jalan Lodaya II/5, Bogor 16151, West Java, Indonesia, Tel./Fax. +62-251-8320417/

8360712, ♥e-mail: witafar@ipb.ac.id; witafar@yahoo.com

2Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB), Bogor 16680, West Java, Indonesia

3Department of Psychology, Center for Global Field Study and Washington National Primate Research Center, University of Washington, Seattle,

Washington 98195-7330, USA

ABSTRACT

Perwitasari-Farajallah D, Kyes RC, Iskandar E (2010) Microsatellite DNA polymorphisms for colony management of long-tailed macaques (Macaca fascicularis) population on the Tinjil Island. Biodiversitas 11: 55-58. Polymorphic genetic markers are the basic requirement for studies on population and conservation genetics of non-human primates. In this paper, we screened microsatellites for their polymorphism and gene typing of DNA samples from blood of wild long-tailed macaques (Macaca fascicularis) from Tinjil Island population. Among the three primer sets tested, two are polymorphic. They were D1S548 and D3S1768. Average observed heterozygosity (Ĥ) within populations ranged between 0.264-0.555. D1S1768 locus was highly polymorphic and 24 alleles were detected among two loci. Estimation of genetic variability for the Tinjil population (Ĥ) was 0.485. The results obtained provide further insight into the long-term viability of the population and help in creating genetic management of both captive and natural habitat breeding colonies of primates.

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